Bacteriophage cultures is rated 46 out of 5 by 9 rated 5 out of 5 by charlotte from bacteriophage (t4r+) perfect for my 5th grader science fair project i will be buying from her again great product for microbiology lab this bacteriophage was very effective for our microbiology lab purposes of infecting ecoli and seeing plaques will.
Objective infect growing e coli b cultures with bacteriophage t4r and demonstrate that the number of plaques formed can vary from a few to innumerable demonstrate that phage can effectively kill e coli and could possibly be used to fight e coli infections.
An experiment to infect e coli b cultures with bacteriophage t4r pages 6 words 1,561 view full essay more essays like this: not sure what i'd do without @kibin - alfredo alvarez, student @ miami university exactly what i needed - jenna kraig, student @ ucla wow most helpful essay resource ever. Stationary phase infections were performed with cultures of e coli zk126 or zk1000 in m9 at a range of multiplicities of infection (moi), as indicated either 5 or 24 h post-infection, nutrients were added to bring the concentration in the infection flask up to 012% w/v glucose and 01% w/v casamino acids.
Today you will infect the bacterium escherichia coli b with its specific bacteriophage, coliphage t4 in the first part of the lab you will perform a plaque count a plaque is a small, clear area on an agar plate where the host bacteria have been lysed as a result of the lytic life cycle of the infecting bacteriophages. Infection of actinomycin-permeable mutants of escherichia coli with urea-disrupted bacteriophage culture ofe coli as19 was diluted 20-fold with infection with t47r e coli band as19 were grown in broth and harvested duringlogarithmic andearly stationary. Which medium was escherichia coli b and t4r mixed soft agar how much escherichia coli b was required 2-3 drops how much t4r bacteriophage was required 1 ml why do you gently rock the plate once you pour the soft agar on the tsa so that the soft agar completely covers the surface.
Using a new sterile 10 ml pipette, add 05 ml (see fig 11) of e coli b to the 01 ml of bacteriophage in each of the 3 tubes from step 3 as shown in fig 9 using a vortex mixer, mix the tube thoroughly. Infection of actinomycin-permeable mutants of escherichia coli with urea-disrupted bacteriophage a a03-ml amount ofovernight culture ofcells wasplatedwith01 mloft4r (original phagetiter: 55 x 107/ml) 100c l 500 x 800 /400x 6002 g x _ 300 0a 5o e z infection with t47r e coli band as19 were grown in broth and.
Rated 5 out of 5 by sheryl from always works as expected every year the virus performs in the experiment as expected, producing plaques on a lawn of e coli b we will continue to use this product we will continue to use this product.
Ppb in synchronous e coli b/r cells pregrown in glucose-minimal medium, infected (at a multiplicity of 05) with bacteriophage t4 + and ∗, eluted “babies” and ×, “old” cells, after 40 min of growth following elution + and , without chloroform ∗ and ×, with chloroform.